Application of biofabrication technologies

Apply biofabrication technologies to study regenerative and degenerative phenomena

With biofabrication platforms, it will be possible in the near future (short-term) to control the differentiation of (stem) cells, and create (medium-term) vascularized and innervated complex organs to better integrate with the surrounding tissues in the implantation site. These constructs could also serve as in vitro 3D models (long-term) to study mechanisms and possible therapies behind pathological conditions (e.g. bone models to study cancer metastasis) or organ regeneration (e.g. pancreas, kidney, glands). In this research line, other enabling technologies will be fundamental for the rationale development of functional constructs (e.g. modeling), and for characterization of the engineered tissues (e.g. imaging, biochemical, mechanical, and molecular analysis). This will be fed back into the design of biofabricated constructs to achieve on one side a better 3D construct, on the other side possible new therapies for targeted diseases. 

Researchers involved in this project

Khadija Mulder, Jip Zonderland, David Gomes, Afonso Malheiro, Rabeil Sakina, Sara Neves, Tianyu Yao, Abishek Harichandan, Paul Wieringa, Carlos Mota, Febriyani Damanik

Related publications

  1. van Kampen K, Olaret E, Stancu IC, Campos DD, Fischer H, Mota C, Moroni L. Hypotrochoidal scaffolds for cartilage regeneration. Materials Today Bio 2023; 23:100830.

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  2. Tabury K, Rehnberg E, Baselet B, Baatout S, Moroni L. Bioprinting of Cardiac Tissue in Space: Where Are We?. Adv Healthc Mater 2023; 12(23): 2203338

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  3. Malheiro A, Thon M, Lourenço AF, Seijas Gamardo A, Chandrakar A, Gibbs A, Wieringa P, Moroni L. A humanized in vitro model of innervated skin for transdermal analgesic testing. Macromolecular Bioscience 2023; 23(1): 2200387

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  4. Caiado Decarli M, Seijas‐Gamardo A, Morgan FLC, Wieringa P, Baker MB, Silva JVL, Moraes AM, Moroni L, Mota C. Bioprinting of Stem Cell Spheroids Followed by Post‐Printing Chondrogenic Differentiation for Cartilage Tissue Engineering. Adv HealthcMater 2023; 12(19): 2203021.

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  5. Camarero‐Espinosa S, Yuan H, Emans PJ, Moroni L. Mimicking the Graded Wavy Structure of the Anterior Cruciate Ligament. Adv Healthc Mater 2023; 12(17): 2203023.

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  6. Camarero-Espinosa S, Moroni L. Janus 3D printed dynamic scaffolds for nanovibration-driven bone regeneration. Nature Comm 2021; 12(1): 1031

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  7. Mota C, Danti S, D'Alessandro D, Trombi L, Ricci C, Puppi D, Dinucci D, Milazzo M, Stefanini C, Chiellini F, Moroni L, Berrettini S. Multiscale fabrication of biomimetic scaffolds for tympanic membrane tissue engineering. Biofabrication. 2015 May 7;7(2):025005

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  8. Camarero‐Espinosa S, Beeren I, Liu H, Gomes DB, Zonderland J, Lourenço AFH, van Beurden D, Peters M, Koper D, Emans P, Kessler P, Rademakers T, Baker MB, Bouvy N, Moroni L. 3D Niche‐Inspired Scaffolds as A Stem Cell Delivery System for The Regeneration of The Osteochondral Interface. Advanced Materials 2024; 16(1): 2310258.

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  9. Marchioli G, van Gurp L, van Krieken PP, Stamatialis D, Engelse M, van Blitterswijk CA, Karperien MB, de Koning E, Alblas J, Moroni L, van Apeldoorn AA. Fabrication of three-dimensional bioplotted hydrogel scaffolds for islets of Langerhans transplantation. Biofabrication 2015, May 28;7(2):025009.

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  10. Hendrikson WJ, Zeng X, Rouwkema J, van Blitterswijk CA, van der Heide E, Moroni L. Biological and Tribological Assessment of Poly(Ethylene Oxide Terephthalate)/Poly(Butylene Terephthalate), Polycaprolactone, and Poly (L\DL) Lactic Acid Plotted Scaffolds for Skeletal Tissue Regeneration. Adv Healthc Mater. 2016 Jan;5(2):232-43.

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  11. Hendriks JAA, Moroni L, Riesle J, de Wijn JR, van Blitterswijk CA. The effect of scaffold-cell entrapment capacity and physico-chemical properties on cartilage regeneration. Biomaterials 2013; 34(17): 4259-65.

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  12. Moroni L, Elisseeff JH. Biomaterials engineered for integration. Materials Today 2008; 11(5): 44-51.

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